The collection of negatives and slides includes almost 50,000 exhibits. The oldest exhibits are the glass negatives created at the turn of the XIX and the XX centuries, owned by Aleksandras Jurašaitis, a photographer and zincographer, as well as the collection of photography enthusiast Father Juozapas Žiogas. The period of Independence of the Republic of Lithuania is represented by the negatives owned by photographers Vincas Ferinauskas, Juozas Daubaras, Jonas Kinčinas, K. Daugėla, and other artists. Later period is reflected by the negatives of K. Baulas, a master of pavilion photography, and the negatives and photographic experiments of an experimental photographer P. Karpavičius. In 2005, the founder of the Photography museum and a photographer Antanas Dilys gave the negatives of his works to the museum. They constitute a separate archive of negatives of the Photography department.